科技服务 SERVICE

Anti-lactyllysine rabbit pAb

  • 服务简介

    Product Description

    Anti-lactyllysine rabbit pAb

    Anti-lactyllysine rabbit pAb

    Figure A: Dot blotting analysis on indicated amount of peptides using anti-Kla rabbit pAb. The list of peptides is included in the table on the right

    Figure B: ?Western blotting analysis on 30?μg?of crude proteins from ?HeLa whole cell with (+) or without (-) treatment of sodium lactate (100 mM, 24hr), 30μg whole ?tissue lysates of mouse kidney and mouse spleen using anti-Kla rabbit pAb (1: 1000).

    Figure C: Western blotting analysis on 30?μg?of crude proteins from human HeLa, mouse N2a and Rat BRL whole cell lysates using anti-Kla rabbit pAb (1: 1000)


    Source/Purification

    This product is produced by immunizing rabbits with lactylated BSA.

    Antibodies are purified by lactylated-lysine agarose affinity chromatography.



    Specificity

    All crotonylated proteins expected

    Recommended Applications

    ELISA, Dot blot, Western blot.? Recommended antibody dilution: WB: 1:1000


    NOTE: For WB, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking. Prepare working dilution immediately before use. Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications


    ?




    Scientific Description

    Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases. Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification, derived from the cellular metabolite lactate.




    Storage & Stability

    The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.




  • 技术原理

    Cat#:

    PTM-1401

    Ab Type:

    P

    Applications:

    WB

    Species:

    All


  • 仪器设备

  • 技术优势

    Zhang D, Tang ZY. et al (2019). Metabolic regulation of gene expression by histone lactylation. Nature, vol. 574, pages 575–580 .

  • 应用领域

    ELISA, WB, IAP. Not tested in other applications

    *WB=Western blotting; IAP: Immuno affinity precipitation


  • 参考文献

相关推荐 SERVICE
  • 非标(Label-free)蛋白质组定量技术是一种不依赖于同位素标记的新型蛋白质定量技术,该技术通过液质联用对蛋白质酶解肽段进行分析,无需昂贵的稳定同位素标签做内部标准,只需分析大规模鉴定蛋白质时所产...
  • 蛋白质磷酸化(Phosphorylation)是指蛋白质在磷酸化激酶的催化下,把ATP或GTP?#31995;?#30967;酸基转移到蛋白质的特定位点(氨基酸残基Ser、Tyr、Thr)?#31995;?#36807;程。磷酸化是一种广泛存在的翻译后...
  • 非标(Label-free)蛋白质组定量技术是一种不依赖于同位素标记的新型蛋白质定量技术,该技术通过液质联用对蛋白质酶解肽段进行分析,无需昂贵的稳定同位素标签做内部标准,只需分析大规模鉴定蛋白质时所产...
  • 蛋白质磷酸化(Phosphorylation)是指蛋白质在磷酸化激酶的催化下,把ATP或GTP?#31995;?#30967;酸基转移到蛋白质的特定位点(氨基酸残基Ser、Tyr、Thr)?#31995;?#36807;程。磷酸化是一种广泛存在的翻译后...
Copyright ?2018-2020 杭州景杰生物科技有限公司
犀牛云提供云计算服务
X
合作
交流
1

QQ设置

3

SKYPE 设置

4

阿里旺旺设置

等待加载动态数据...

等待加载动态数据...

5

电?#26114;?#30721;管理

  • 400-100-1145
6

二维码管理

等待加载动态数据...

等待加载动态数据...

展开
合作交流
填写信息,我们的专属学术顾问将为您解答疑问!
安徽福彩快三走势图
新手游戏直播平台哪个赚钱 自己赚钱自己花 不用老公的钱 赚钱和情怀哪个重要 大屏导航机不赚钱 紫金矿业股票分析 梦幻西游分解60铁赚钱 最赚钱男演员 002647股票分析 过年在景区怎么赚钱 大宗商品股票指数 60魔兽盗贼怎么赚钱 网上打麻将赚钱的 谁有好股票推荐 优酷视频赚钱佣金多少钱 趣头条 怎么赚钱 网上兼职赚钱是否可靠