科技服務 SERVICE

Acetyl-p53 (Lys139) mouse mAb

  • 服務簡介

    Source/Purification:??

    This product is produced by immunizing mice with a synthetic acetyl peptide corresponding to residues surrounding Lys139 of human p53. Antibodies are purified by protein G-conjugated agarose followed by

    acetylated p53 (Lys139) peptide affinity chromatography.


    Recommended Applications:?

    ELISA, Dot blot, Western blot.? Recommended antibody dilution: WB: 1:2000

    NOTE: For WB, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking. Prepare working dilution immediately before use. Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications


    ? ? ? ? ? ? ? ? ? ? ? ? ? ?

    Scientific Description:

    ?

    Acetyl-p53 (Lys139) mouse mAb

    Figure A: Dot blotting analysis on indicated amount of peptides using anti-p53K139ac mouse mAb. The list of peptides is included in the table on the right.

    Acetyl-p53 (Lys139) mouse mAb

    Figure B: Western blotting analysis on 30 μg of crude proteins from human MCF-7 whole cell lysates with (right) or without (left) treatment of etoposide (15ug/ml, 24 hours) and (500ng/ml, 24 hours)Trichostatin A using acetyl-p53 (Lys139) mouse mAb (1: 2000).

    Acetyl-p53 (Lys139) mouse mAb

    Figure C: Western blotting analysis on 30 μg of crude proteins from mouse brain and mouse liver whole tissue lysates using acetyl-p53 (Lys139) mouse mAb (1: 2000).


    Storage & Stability:

    The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.

  • 技術原理

    Cat#:

    PTM-185

    Ab?Type:

    M

    Application:

    WB, Dot, ELISA

    Species:

    Human, Mouse, Rat,?

    Price: (100ul)

    3060

    Price: (20ul)

    700

  • 儀器設備

  • 技術優勢

  • 應用領域

    ELISA, Dot blot, Western blot.?

    Recommended antibody dilution: WB: 1:2000



  • 參考文獻

相關推薦 SERVICE
  • 非標(Label-free)蛋白質組定量技術是一種不依賴于同位素標記的新型蛋白質定量技術,該技術通過液質聯用對蛋白質酶解肽段進行分析,無需昂貴的穩定同位素標簽做內部標準,只需分析大規模鑒定蛋白質時所產...
  • 蛋白質磷酸化(Phosphorylation)是指蛋白質在磷酸化激酶的催化下,把ATP或GTP上的磷酸基轉移到蛋白質的特定位點(氨基酸殘基Ser、Tyr、Thr)上的過程。磷酸化是一種廣泛存在的翻譯后...
  • 非標(Label-free)蛋白質組定量技術是一種不依賴于同位素標記的新型蛋白質定量技術,該技術通過液質聯用對蛋白質酶解肽段進行分析,無需昂貴的穩定同位素標簽做內部標準,只需分析大規模鑒定蛋白質時所產...
  • 蛋白質磷酸化(Phosphorylation)是指蛋白質在磷酸化激酶的催化下,把ATP或GTP上的磷酸基轉移到蛋白質的特定位點(氨基酸殘基Ser、Tyr、Thr)上的過程。磷酸化是一種廣泛存在的翻譯后...
Copyright ?2018-2020 杭州景杰生物科技有限公司
犀牛云提供云計算服務
X
合作
交流
1

QQ設置

3

SKYPE 設置

4

阿里旺旺設置

等待加載動態數據...

等待加載動態數據...

5

電話號碼管理

  • 400-100-1145
6

二維碼管理

等待加載動態數據...

等待加載動態數據...

展開
合作交流
填寫信息,我們的專屬學術顧問將為您解答疑問!
安徽福彩快三走势图
广西体育彩票11选55 大乐透开奖走势图 福建36选7机选 车联网概念股 山西扣点点麻将下载 江西11选5开奖号 北京好的期货配资公司 澄星股份股票行情查 2019陕西麻将1元微信群 老快3老快3投注 山东11选5开奖统 今日贵州快3开奖 幸运3D走势图 大众麻将的打法 股票开户营业部 天才麻将少女漫画