%PDF-1.5 %忏嫌 1 0 obj <>/OCGs[9 0 R 10 0 R 11 0 R 12 0 R 13 0 R 14 0 R 15 0 R 16 0 R 17 0 R 18 0 R]>>/Pages 3 0 R/Type/Catalog>> endobj 2 0 obj <>stream Adobe Illustrator CC 22.0 (Windows) 2019-05-13T15:39:06+09:00 2019-05-13T15:39:06+08:00 2019-05-13T15:39:06+08:00 3 sRGB IEC61966-2.1 100 渭l at supplied in liquid form 100 渭l at supplied in liquid form Species reactivity is determined by WB. Kept at -20掳C after rec Species reactivity is determined by WB. Kept at -20掳C after reconstituted. 宸笂琛头1 Cat#: Species: Antibody type: Size: Form: Size: Form: sentence4 Western blotting analysis on 30 碌g of crude proteins extracted from HeLa cells with (right) or without (left) sodium vanadate treatment (1 mM for 15 mins) using anti-phosphotyrosine rabbit pAb. (1:1000). sentence3 Dot blotting analysis on indicated amount of peptides using Anti-phosphotyrosine rabbit pAb. The list of peptides is included in the table. sentence2 The rabbit polyclonal antibody is produced by immunizing rabbits with synthetic phospho-Tyr-containing peptides and immunoaffinity purified by protein A followed by phosphotyramine affinity chromatography. sentence1 Anti-rabbit secondary antibodies must be used to detect this antibody. Molecular-Weight Multiple Molecular Wt. Molecular Wt. Immunogen Phosphotyramine -KLH #Immunogen Immunogen Species-Reactivity All species #Species Reactivity Species Reactivity Applications WB #Applications Applications Uniprot-ID / #Uniprot ID: Uniprot ID: Clone-Number # Ab-type Polyclonal Rabbit Rabbit 0.4 mg/ml 0.4 mg/ml PTM-702 PTM-702 Antibody-name Anti-phosphotyrosine rabbit pAb Order: order@ptm-biolab.com; Support: 400-100-1145 service@ptm- Order: order@ptm-biolab.com; Support: 400-100-1145 service@ptm-biolab.com; Web: www.lzeti.club; PTM Bio, lnc. Figure B: Western blot Figure B:Western blot Figure A:Dot blot Figure A:Dot blot Specificity: Specificity: Recommended Application: ELISA, Dot blot, Western blot. Reco Recommended Application: ELISA, Dot blot, Western blot. Recommended antibody dilution: WB: 1:1000 NOTE: For WB, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking. Prepare working dilution immediately before use. Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications. Source & Purification: Source & Purification: Technical Support Technical Support Academic Platform Academic Platform Storage & Stability: Storage & Stability: The antibody is kept in PBS with 50% glycerol and 0.01% sodium The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20掳C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage. Schematic diagram for reconstitution instructions Schematic diagram for reconstitution instructions 12,000 x g 20 seconds 12,000 x g 20 seconds Cut the pipette tip Cut the pipette tip Pipet up and down multiple times Pipet up and down multiple times Phosphorylation and dephosphorylation Phosphorylation and dephosphorylation Scientific Background: Tyrosine phosphorylation is the addition Scientific Background: Tyrosine phosphorylation is the addition of a phosphate group to the amino acid tyrosine on a protein. It is one of the main types of protein phosphorylation. This transfer is made possible through enzymes called tyrosine kinases. Tyrosine phosphorylation is one of the major means of cell signaling transduction in multiple pathophysiological conditions regulated by tyrosine kinase activities. In cancer, unregulated tyrosine kinase activity can drive tumor malignancy and formation by generating inappropriate proliferation and survival signals. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to these studies. The anti-phosphotyrosine antibody (clone 3B6) developed by PTM Biolabs, Inc. provides an exceptionally sensitive tool for studying tyrosine phosphorylation and its kinase activities in biomedical and preclinic studies as well as high throughput drug discovery. 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